This antibody does not crossreact with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. We use cookies to enhance the usability of our website. Select, antibody name, antigen gene name, isotype balbc mice, cellular binding site, recommended dilution, anatomical structure labeled, availability. Reliability score i a reliability score is manually set for all genes and indicates the level of reliability of the analyzed protein expression pattern based on available rnaseq data, proteingene characterization data and immunohistochemical data from one or several antibodies with non. Avian, bovine, canine, chicken, equine, feline, guinea pig, human, mammal, mouse, porcine, primate, rabbit, rat, sheep. What is the best protocol for the use of gfap on a rat. Intermediate filaments form networks that provide support and strength to cells.
Gfap astrocytopathy associated with meningitis, encephalitis, or myelitis expands the spectrum of autoimmune antibodyassociated central nervous system cns. After adding the product to the cart by clicking the button below you will see if there is a volume discount. Briefly, standards, quality controls qclow and qchigh, and serum samples were incubated in microplate wells precoated with polyclonal antihuman gfap antibody for 2 hours. Orders fi 877616cell 2355 support fi 877678tech 8324 web fi. Recombinant human gfapgfap, a classiii intermediate filament, is a cellspecific marker that, during the. How reliable is gfap as a marker of structuralmorphological.
Skretting is now offering our premium marine hatchery diet gemma micro, through our new webshop. Zebrafish have astrocytes 23, and zebrafish gfap shares 67% identity and 77%. P0809, p0810 and p1691 are blocking peptides that are not suitable for use with nb10053809. Gfap antibody staining protocol for immunohistochemistry. It is aimed to help labome visitors find the most suited gfap antibody. Gfap antibody gf5 is a high quality mouse monoclonal recommended for detecting 4345 kd fragment of gfap of mouse, rat and human origin by wb, ip and if. Doubleimmunofluorescence with antibodies against glial fibrillary acidic protein gfap and c4 demonstrated. Immunohistochemical labeling using antibodies against the glial fibrillary acidic protein gfap, a common astrocyte and radial glia marker. Glial fibrillary acidic protein gfap is an intermediate filament protein of 52kd found in glial cells such as astrocytes and. Aggregationprone gfap mutation in alexander disease validated. Recombinant human gfapgfap, a classiii intermediate filament, is a cellspecific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Due to their striking genomic similarity to humans, regenerative abilities. Zebrafish antibodies primary antibodies sigmaaldrich. Gfap antibody 2e1 is available as both the nonconjugated anti gfap antibody form, as well as multiple conjugated forms of anti gfap antibody, including agarose, hrp, pe, fitc and multiple alexa fluor conjugates.
Please note that antibodies should be stored in a 80c freezer if they arrive frozen. Localization of nerve growth factor, neurotrophin3, and glial cell linederived neurotrophic factor in nestinexpressing reactive astrocytes in the caudateputamen of 1methyl4phenyl1,2,3,6tetrahydropyridinetreated c57bl mice. This protein is a member of the intermediate filament family of proteins. Antibody z0334 glial fibrillary acidic protein gfap by dako. Immunofluorescent analysis of gfap in human ipscderived astrocytes. Anti gfap antibody products anti gfap antibodies are offered by a number of suppliers. It is a type iii intermediate filament if protein that is expressed by numerous cell types of the central nervous system cns, including astrocytes and ependymal cells during development. Glial fibrillary acidic protein gfap is a protein that is encoded by the gfap gene in humans. Jo1 protein his tag tpo protein his tag glp1 antibody. Glial fibrillary acidic protein gfap is used as a marker for retinal and. I have found that my gfap goat antigfap from abcam antibody was sensitive to the length of time i post. In the peripheral nervous system, gfap has been demonstrated in schwann cells, enteric glial cells and satellite cells of human. Glial fibrillary acidic protein gfap antibody fitc abbexa. For in vitro diagnostic use glial fibrillary acidic protein gfapm.
Zirc monoclonal antibodies zebrafish international. Glial fibrillary acidic protein gfap m concentrated and prediluted monoclonal antibody 901065042519 rev. Glial fibrillary acidic protein gfap dako m0761 product. Antibody staining consistent with rna expression data. Adult neurogenesis arises from niches that harbor neural stem cells nsc.
How reliable is gfap as a marker of structuralmorphological changes in astrocytes. For in vitro diagnostic use glial fibrillary acidic protein gfap m ga5 is a mouse monoclonal antibody that is intended for laboratory use in the qualitative. Adequate amount of material to conduct 10mini western blots. Antibody gfap glial fibrillary acidic protein gfap by aves labs. Regulatory elements that direct the glialspecific expression of zebrafish gfap were identified in genomic sequences from the zebrafish gfap gene. Glial fibrillary acidic protein, or gfap, is an intermediate filament if protein belonging to the type iii subclass of if. Zebrafish astroglial morphology in the olfactory bulb is. Storage aliquot the gfap antibody and store frozen at 20oc or colder. Gfap is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in nonmyelinating schwann cells of peripheral nerves. In vivo single cell analysis reveals distinct behavior of neural stem.
Gfappositive radial glial cells are an essential progenitor. It has also been detected in the glial cells of the enteric nervous system. Glial fibrillary acidic protein gfap is an intermediate filament protein of 52kd found in glial cells such as astrocytes and ependymal cells. The third was a monoclonal antibody zrf1, kindly provided by m. Clicking the images or links will redirect you to a website hosted by benchsci that provides thirdparty scientific content. Glial fibrillary acidic protein gfap, as a member of the cytoskeletal protein family, is the principal 89 nm intermediate filament in mature astrocytes of the central nervous system cns. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. Due to a lack of a working antibody against zebrafish nestin and to assess the degree of correlation between tg. The cells were fixed with 4% paraformaldehyde for 15 min at room temperature, and then permeabilized and blocked with 0.
Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Gfap elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Next we examined the expression of glial fibrillary acidic protein gfap by immunostaining with antigfap monoclonal antibody, zrf1. Antigfap antibody products antigfap antibodies are offered by a number of suppliers. If thawed upon arrival, please keep antibodies in the refrigerator at 4c. Gfap antibody ga 5 is a high quality mouse monoclonal recommended for detecting gfap of h, r and porcine origin by wb, ip, if and ihcp. Gfap antibody this mouse monoclonal gfap antibody clone 6a6 is one of our most popular neuroscience antibodies.
Gfap antibody fitc is a rabbit polyclonal antibody against gfap conjugated to fitc. Gtx128741 wb image gfap antibody detects gfap protein by western blot analysis. After incubation with the chicken antigfp antibody, a solution of biotinylated anti chicken antibody cat. Gemma micro provides complete nutrition for all life stages.
Please use this form to recommend updates to the information in zfin. It is specific for human gfap and has been validated for use in elisa, immunofluorescence, immunohistochemistry and western blot applications. Expression of glial fibrillary acidic protein and its. The gfap gene provides instructions for making a protein called glial fibrillary acidic protein.
Z0334 glial fibrillary acidic protein gfap dako citeab. Intermediate filaments of zebrafish retinal and optic nerve astrocytes. Glial fibrillary acidic protein gfap hytest web store. This target gene encodes the protein glial fibrillary acidic protein in humans and may also be known as alxdrd. What is the best protocol for the use of gfap on a rat retina in order to identify glial cells. Localization of nerve growth factor, neurotrophin3, and glial cell linederived neurotrophic factor in nestinexpressing reactive astrocytes in the caudateputamen of. A monoclonal antibody stains radial glia in the adult zebrafish. We employed two novel transgenic zebrafish lines and endogenous markers. Oct 12, 2015 briefly, standards, quality controls qclow and qchigh, and serum samples were incubated in microplate wells precoated with polyclonal antihuman gfap antibody for 2 hours, followed by 1 hour incubation with a biotinlabeled monoclonal antihuman gfap antibody solution and 1 hour incubation with a streptavidinhorseradish peroxidase conjugate. Glial fibrillary acidic protein gfapm concentrated and prediluted monoclonal antibody 901065042519 rev. Monoclonal antibody for studying gfap in the neuroscience research area. We appreciate as much detail as possible and references as appropriate.
Autoimmune gfap astrocytopathy associated with meningitis. Can i use your gfap antibody nb10053809 to detect p0809, p0810 and p1691 gfap proteins. Zebrafish protein an overview sciencedirect topics. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Glial fibrillary acidic protein gfap belongs to the class iii of the intermediate filament proteins highly specific to astrocytes in the. This gfap antibody was produced from a rabbit immunized with a klh conjugated synthetic peptide between 236amino acids from the nterminal region of zebrafish gfap. It is a type iii intermediate filament if protein that is expressed by numerous cell types of the central nervous. Gfap glial fibrillary acidic protein gfap aves labs.
I plan to use a polyclonal rabbit anti gfap as my primary antibody and goat antimouse serum as. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases. Ca with appropriate second antibodies conjugated respectively to tritc and cy5. Gfap is a monomeric molecule with a molecular mass between 40 and 53 kda and an isoelectric point between 5. After adding the product to the cart by clicking the button below you will see if there is a volume discount available. What is the best protocol for the use of gfap on a rat retina. I plan to use a polyclonal rabbit antigfap as my primary antibody and goat antimouse serum as. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to. Gfap is a marker of radial glial cells and astrocytes, both of which. Neither the content nor the benchsci technology and processes for selection have been evaluated by us. Also, current batches of zirc antibody may have dilution rates that vary from the dilution rates found in publications. It has also been detected in the glial cells of the enteric nervous system and some schwann cells in the peripheral nervous systems. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. In zebrafish, onr is rapid neurites cross the lesion and enter the.
A monoclonal antibody stains radial glia in the adult zebrafish danio rerio. Our gfap antibodies can be used in a variety of model species. Gfap glial fibrillary acidic protein is a member of the class iii intermediate filament protein family. I am interested in native or recombinant gfap protein without gst tag or in urea buffer. Cereblon control of zebrafish brain size by regulation of. About these antibodies all antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al.
Glial fibrillary acidic protein human elisa gfap biovendor. Recommended that the undiluted antibody be aliquoted into smaller working volumes 1030 ulvial depending on usage upon arrival and stored long term at 20 c or 80 c, while keeping a working aliquot stored at 4 c for short term. Summary of gfap flj45472 expression in human tissue. Similar refinements in the zebrafish data returned 1248 proteins with 2344 ppis in. Gfap is expressed exclusively in astrocytes in the central nervous system. Glial fibrillary acidic protein gfap is the predominant component of astrocyte intermediate filaments in the central nervous system. Recommended that the undiluted antibody be aliquoted into smaller working volumes.
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